Western blot was performed using SLC25A11 Polyclonal Antibody (Product # PA5-27510) and a 30 kDa band corresponding to SLC25A11 was observed across cell lines. Whole cell extracts (40 µg lysate) of MCF7 (Lane 1), Hep G2 (Lane 2), HeLa (Lane 3), Mouse heart (Lane 4) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX), 10 well. Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20,000 dilution) using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Atto Ultimate Sensitivity Substrate (Product # A38556).